Birjand University of Medical Sciences< Iran
Title: Rapid Green Synthesis of Gold Nanoparticles Using Crocetin and their Anticancer Activity
Dr. Hoshyar is working as an Assistanat Professor at the Cellular & Molecular Research Center, Birjand University of Medical Sciences, Iran
Gold nanoparticles (AuNPs) have been used in various mechanisms including drug delivery, cancer diagnosis and treatment due to their unique chemical and physical properties. In this study, we applied one of main caratenoids of saffron stigma, crocetin, as reducing agent for one-pot green synthesis of controlled size AuNPs for the first time. To optimize the varoius factors on size and amount of produced AuNPs, different concentrations of gold ions (prepared from HAuCl4, 0–5mM) and crocetin (200-500 mM) were used at various time (1h–10 weeks) and temperatures (25–75 °C). These AuNPs were characterized through UV–vis, TEM and XRD techniques. The anti-cancer effect of AuNPs on breast cancer cells was determined using MTT assay. Optimal temperature and pH for biosynthesis of AuNPs conjugated with this antioxidant were 50ºC and 7.5, respectively. XRD and TEM results were authorized AuNPs production in 5-10 nm and stable, spherical and uniform. The cellular data were showed that these AuNPs significantly decreased cancerous cells' growth after 24 and 48hours in a time- and dose-dependent manner (P<0.05). Our study suggest that such AuNPs can be synthesized simply and quickly with invaluable clinical as well as pharmaceutical properties which can help to treat human breast cancer.