Shahzad Ali Shahid Chatha
University of Faisalabad, Pakistan
Title: Characterization of nutritionally essential minor saccharides in escalating cultivars of daucus carota by hplc-ri detector
Biography
Biography: Shahzad Ali Shahid Chatha
Abstract
Carbohydrates in Daucus carota prove its legitimacy, sensory and nutritional properties. The objective of present study was to quantify the minor sugars, in addition to major saccharides in new lines of D. carota by developing and validating a simple analytical method using high performance liquid chromatography with refractive index detector (HPLC-RI). Sample preparation required only water-blending, filtration, demineralization and degassing prior to injection. The concentration (p < 0.05) of total sugars in terms of fructose, glucose, maltose, maltotriose and maltodextrin in selected cultivars (DCW, DCY, DCP, T29, DCR, DC3 and DC90) were 4.511, 5.165, 6.311, 5.281, 4.912, 5.099 and 4.448g/100g of fresh weight (FW) respectively. The sugars types and concentrations were confirmed by retention times and peak areas of standards. Validation parameters proved that developed method is efficient state of art having correlation coefficients (> 0.999). Limits of detection and quantification are consistent which were in the range of 48.13 to 59.45 mg/L and 49.36 to 178.23 mg/L respectively. Recovery of sugars was > 90%. Developed method was applicable to quantify all types of sugarsinD. carota. DCP cultivar contained higher concentration of sugars, it may be recommended in process industries for the extraction of dietary sugars of vegetal origin.