Yahya Shafiei Bavil Oliaei
Islamic Azad University, khoy, Iran
Title: Microencapsulation of Lactobacillus plantarum with alginate/resistant-starch and development of a new method for evaluating the viability of entrapped bacteria
Biography
Biography: Yahya Shafiei Bavil Oliaei
Abstract
This study aimed to evaluate the emulsion technique for microencapsulation of L. plantarum using alginate/resistant-starch mixed gel and to develop a new method for evaluating the viability of entrapped bacteria and their release process. A mixture of sodium alginate (2% w/v) and resistant starch (2% w/v), containing bacterial suspension (1% v/v) was used in microencapsulation. The morphology of microcapsules was studied using Scanning Electron Microscopy (SEM). The viability of the entrapped bacteria as well as their ability to release was studied using light microscopy. A wet-mount smear of microcapsules was prepared, slightly stained by Lugol’s iodine, and studied before and after releasing at the presence of Na+ ions. The stability of microcapsules in Bile Salts Solution (BSS), Simulated Gastric Juice (SGJ), Pancreatin Enzymes Solution (PES), and Phosphate Buffer Solution (PBS) were studied with and without 400 rpm mechanical shaking. The prepared microcapsules were spherical, with the mean size of 14.84 μm, containing 1.7×109 cfu g-1 viable cells. Direct microscopic observations indicated that each microcapsule contained one or more living bacterial cells. Bacterial cells were weekly stained and their brownian motion could be traced inside the microcapsules and after being released. The stability of microcapsules was respectively 60 and 90 min. in BSS and PES without mechanical shaking, and 30 min. at rest conditions. The present study indicated that the emulsion technique is an efficient method for microencapsulation of L. plantarum and the new suggested method could be successfully used for evaluating the viability of entrapped bacteria and their release process.